Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fibrinogen-mediated epidermal cell migration: structural correlates for fibrinogen function.

D J Donaldson1, J T Mahan, D Amrani

  • 1Department of Anatomy and Neurobiology, Health Science Center, University of Tennessee, Memphis 38163.

Journal of Cell Science
|September 1, 1989
PubMed
Summary

Epidermal cells use fibrinogen (FGN) for migration during wound healing. Specific structural features of FGN, particularly the D1 fragment and A alpha chains, are crucial for this cellular response.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced Surface Partitioning of Nitrate Anion in Aqueous Bromide Solutions.

The journal of physical chemistry letters·2015
Same author

Reply to "Comment on 'Photolysis of Polycyclic Aromatic Hydrocarbons on Water and Ice Surfaces' and on 'Nonchromophoric Organic Matter Suppresses Polycyclic Aromatic Hydrocarbon Photolysis in Ice and at Ice Surfaces'".

The journal of physical chemistry. A·2015
Same author

New paradigms in sepsis: from prevention to protection of failing microcirculation.

Journal of thrombosis and haemostasis : JTH·2015
Same author

Heterogeneous photochemistry in the atmosphere.

Chemical reviews·2015
Same author

Red-light initiated atmospheric reactions of vibrationally excited molecules.

Physical chemistry chemical physics : PCCP·2013
Same author

Heterogeneous photooxidation of fluorotelomer alcohols: a new source of aerosol-phase perfluorinated carboxylic acids.

Environmental science & technology·2013

Area of Science:

  • Cell Biology
  • Biochemistry
  • Wound Healing Research

Background:

  • Epidermal cells utilize fibrinogen (FGN) as a substratum for migration during wound closure.
  • Understanding the specific structural components of FGN responsible for this migratory function is essential.

Purpose of the Study:

  • To identify the structural features of fibrinogen (FGN) that facilitate epidermal cell migration.
  • To elucidate the molecular mechanisms underlying FGN-mediated cell migration in wound healing.

Main Methods:

  • Implantation of modified fibrinogen (FGN) coated glass pieces into newt skin wounds.
  • Assessment of epidermal cell migration on native, fragmented, and chemically altered FGN substrates.
  • Utilized purified FGN fragments (D1, E, A alpha, gamma chains) and peptides (RGDS) to probe binding interactions.

Related Experiment Videos

Main Results:

  • Fibrinogen (FGN) coatings supported significantly more epidermal cell migration than BSA.
  • Heat-denatured FGN and Fraction I-9 (lacking a C-terminal segment of A alpha chain) were as effective as intact FGN.
  • Migration occurred on the D1 fragment but was less efficient than on intact FGN; D3 fragment showed reduced activity.
  • Purified A alpha chains supported migration, while gamma chains did not; RGDS peptide blocked migration on intact FGN.

Conclusions:

  • Specific domains within fibrinogen (FGN), including the D1 fragment and A alpha chains, are critical for epidermal cell migration.
  • Integrin-type receptors likely mediate FGN-directed cell migration via RGD-binding sites.
  • These findings provide insights into the molecular basis of wound closure and tissue regeneration.