Chromatography of peptides on a multi-coil counter-current chromatograph

A modified horizontal flow-through coil planet centrifuge has been constructed that has the potential for preparative chromatography. The multi-coil counter-current chromatograph, equipped with a set of four multi-layer coils, has have improved performance with polar solvents suitable for peptide elution. Separations of a group of dipeptides and purification of a cholecystokinin fragment and an undecapeptide were achieved with n-butanol-acetic acid-water at high flow-rates with good resolution.