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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
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Detection of methicillin-resistant Staphylococcus pseudintermedius ST169 and novel ST354 SCCmec II-III isolates

K Ishihara1, A Koizumi2, M Saito2

  • 1Department of Veterinary Medicine,Tokyo University of Agriculture and Technology,Fuchu,Tokyo,Japan.

Epidemiology and Infection
|July 4, 2015
PubMed

Insights

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) in Japanese dogs revealed diverse clonal lineages, including ST71 and related types, challenging previous assumptions about MRSP SCCmec II-III strains. Novel MRSP SCCmec V clones were also identified.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Molecular Epidemiology

Background:

  • Methicillin-resistant Staphylococcus pseudintermedius (MRSP) poses a growing threat to animal and human health.
  • Specific MRSP clonal lineages (ST71-SCCmec II-III, ST68-SCCmec V) have disseminated globally.

Purpose of the Study:

  • To investigate the molecular characteristics of MRSP isolates from dogs in Japan.
  • To compare Japanese MRSP strains with international isolates.

Main Methods:

  • Multilocus sequence typing (MLST) using seven housekeeping genes.
  • Staphylococcal cassette chromosome mec (SCCmec) typing.
  • Detection of antimicrobial resistance genes, including tetM.

Main Results:

  • Three related clonal lineages (ST71, ST169, ST354) were identified within MRSP SCCmec II-III isolates from Japan.
  • The tetM gene was prevalent in ST169 and ST354 SCCmec II-III isolates.
  • MRSP SCCmec V isolates in Japan exhibited diverse sequence types (STs), distinct from previously reported European and North American clones.

Conclusions:

  • Japanese MRSP SCCmec II-III isolates belong to ST71 and related lineages (ST169, ST354), indicating greater diversity than previously understood.
  • The study identified a variety of MRSP SCCmec V clones in Japan, including novel ones.
Keywords:
JapanMLSTStaphylococcus pseudintermediusmethicillin resistance

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