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Minimal andrological requirements for in-vitro fertilization
H H Riedel1, F Hübner, S C Ensslen
1Department of Obstetrics and Gynaecology, RWTH Aachen, FRG.
Human Reproduction (Oxford, England)
|November 1, 1989
Summary
Semen for in-vitro fertilization (IVF) requires specific quality standards, including sperm concentration, motility, and morphology. Microbial contamination must be controlled, with mandatory partner treatment if pathogens are found, to ensure successful human oocyte fertilization.
Area of Science:
- Reproductive Biology
- Andrology
- In-Vitro Fertilization
Background:
- Semen quality is critical for successful in-vitro fertilization (IVF) of human oocytes.
- Minimum requirements for ejaculate parameters are essential for optimizing fertilization rates.
Purpose of the Study:
- To define the minimum quality requirements for human ejaculate used in IVF.
- To evaluate the utility of specific sperm function tests in IVF program acceptance.
Main Methods:
- Experimental investigations to determine optimal semen parameters.
- Bacteriological analysis of sperm preparations.
- Assessment of the hamster oocyte penetration test and the Jeyendran swell test.
Main Results:
- Minimum requirements: >5 million spermatozoa/microliter, ≥30% progressive motility, ≥30% normal morphology.
- Pathogenic microorganisms must be <10(4) in sperm preparations; positive findings necessitate partner treatment.
- Hamster oocyte penetration test offers limited value; swell test shows good correlation with human oocyte fertilization.
Conclusions:
- Strict semen quality criteria are necessary for successful human IVF.
- Microbial control and partner treatment are crucial when pathogens are detected.
- The Jeyendran swell test may be a valuable addition to IVF assessment protocols.