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Updated: Apr 7, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Nano-MeDIP-seq Methylome Analysis Using Low DNA Concentrations.

Lee M Butcher¹, Stephan Beck²

¹UCL Cancer Institute, University College London, London, WC1E 6BT, UK. l.butcher@ucl.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|July 4, 2015

Summary

DNA methylation, an epigenetic mark crucial for development, can now be analyzed genome-wide using nano-MeDIP-seq. This method enables methylome analysis with minimal DNA input, advancing epigenetic studies.

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Area of Science:

Epigenetics
Genomics
Molecular Biology

Background:

DNA methylation is a critical epigenetic mark essential for mammalian development, regulating gene expression by locking in chromatin states.
The methylome, encompassing DNA methylation patterns across the genome, defines cellular identity and influences phenotypic plasticity and disease susceptibility.
Genome-wide analysis is necessary to understand differential methylation patterns impacting complex diseases.

Purpose of the Study:

To introduce and detail nano-MeDIP-seq, a novel method for comprehensive methylome analysis.
To enable methylome profiling using significantly reduced DNA input quantities (nanograms).
To provide a cost-effective and efficient approach for population epigenetic studies.

Main Methods:

Keywords:

Bioinformatics DNA methylation Epigenetics Low concentration Next-generation sequencing Whole genome

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Last Updated: Apr 7, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Methylated DNA Immunoprecipitation

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Utilizes methylated DNA immunoprecipitation (MeDIP) with next-generation sequencing (MeDIP-seq).

Employs an antibody against 5-methylcytosine to capture methylated DNA fragments.

Details the nano-MeDIP-seq protocol for analyzing nanogram quantities of starting material.

Main Results:

Successfully demonstrates methylome analysis using nanogram quantities of DNA.
Highlights the cost-effectiveness and coverage advantages of MeDIP-seq for population studies.
Provides a detailed protocol for the nano-MeDIP-seq technique.

Conclusions:

Nano-MeDIP-seq offers a powerful and accessible tool for genome-wide methylome analysis.
The method complements or replaces array-based methods, particularly for large-scale epigenetic studies.
Facilitates deeper understanding of DNA methylation's role in development, cellular identity, and disease.