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Use of Nonradioactive Detection Method for North- and South-Western Blot
Claudia Franke1, Daniel Gräfe, Holger Bartsch
1Institut für Immunologie, Carl Gustav Carus Technische Universität Dresden, Germany, Fetscherstrasse 74, Dresden, 01307, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|July 4, 2015
Abstract:
Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here we describe the use of a North-western and South-western blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
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