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Updated: Apr 7, 2026
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
Published on: September 26, 2020
Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
Fangmao Ye1, Polina B Smith, Daniel T Chiu
1Department of Chemistry, University of Washington, 36 Bagley Hall, Seattle, WA, 98195, USA.
Abstract:
We demonstrate ultrasensitive fluorescence imaging of proteins on dot blots and western blots using a bright, compact, and orange-emitting semiconducting polymer dot (CNPPV). We achieved a detection limit at the single-picogram level in dot blots; with conventional western blot, we detected 50 pg of transferrin and trypsin inhibitor after SDS-PAGE and transfer onto a PVDF membrane. In this protocol, we describe the preparation of Pdots and its streptavidin bioconjugate and the procedures of dot blot and western blots.
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02:57Southern Blot
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