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Serum Lysozyme (Muramidase) Levels in Intra-Abdominal Abscesses: An Experimental Study

Mathew P Daniel1, Vinay Gaikwad1, Mohan Verghese1

  • 1Department of Surgery, Christian Medical College and Hospital, Brown Road, Ludhiana, 141008 Punjab India.

The Indian Journal of Surgery
|July 4, 2015
PubMed

Insights

Serum lysozyme (muramidase) levels significantly increased in mice with intra-abdominal abscesses. This finding suggests lysozyme

Area of Science:

  • Biochemistry
  • Immunology
  • Surgical Pathology

Background:

  • Intra-abdominal abscesses present a significant diagnostic challenge.
  • Serum lysozyme (muramidase), a bacteriolytic enzyme found in leukocytes, is investigated for its diagnostic potential.
  • Accurate and timely diagnosis of intra-abdominal sepsis is crucial for effective patient management.

Purpose of the Study:

  • To evaluate the role of serum lysozyme concentrations as a diagnostic marker for intra-abdominal abscesses.
  • To assess the sensitivity and specificity of serum lysozyme in detecting intra-abdominal sepsis in a murine model.

Main Methods:

  • Serum lysozyme levels were measured using a standard turbidimetric method.
  • Three groups of murine models were studied: caecal ligation and puncture (CLP)-induced abscess, sham operation, and controls.
  • Statistical analysis was performed to compare lysozyme levels between groups.

Main Results:

  • Mice with experimentally induced intra-abdominal abscesses exhibited significantly elevated serum lysozyme levels compared to sham-operated and control groups (p < 0.001).
  • The study demonstrated high sensitivity and specificity of serum lysozyme for detecting intra-abdominal abscesses in the murine model.
  • Elevated lysozyme levels correlated with the presence of intra-abdominal sepsis.

Conclusions:

  • Serum lysozyme shows promise as a sensitive and specific biomarker for intra-abdominal abscesses in mice.
  • Further clinical studies are warranted to validate the utility of serum lysozyme as a diagnostic adjunct in human patients.
  • Lysozyme may serve as a valuable tool in the early detection of intra-abdominal sepsis.
Keywords:
Intra-abdominal abscessesLysozymeMuramidase

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