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Updated: Apr 7, 2026

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
Published on: April 7, 2023
Back to 2D Culture for Ground State of Intestinal Stem Cells
Mariko Shimokawa1, Toshiro Sato1
1Department of Gastroenterology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan.
Abstract:
Generating highly uniform clonogenic stem cell populations has been remarkably difficult with stem cells from human small and large intestine. A recent report by Wang et al. (2015) demonstrated homogeneous expansion of human fetal intestinal stem cells, providing a new culture system to understand self-renewing mechanisms of intestinal stem cells.
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