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Updated: Apr 7, 2026
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
Published on: September 30, 2019
A CD44 specific peptide developed by phage display for targeting gastric cancer
Dan Zhang1, Huan Jia2, Yan Wang3
1Department of Gastroenterology, The First Affiliated Hospital of Medical School, Xian Jiaotong University, Xi'an, 710061, China. zhangdan.124@stu.xjtu.edu.cn.
Objective:
To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity.
Results:
A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most highly selective peptide, which termed RP-1. Immunofluorescence and flow cytometry analysis indicated that the consensus peptide RP-1 could bind to CD44-positive gastric cancer cells with mean fluorescence intensities significantly higher than that of CD44-negative cells. CD44 knockdown led to decreased binding activity of RP-1 to the same cell line. Tissue array technique was used to identify the relationship (r = 0.556) between peptide binding and CD44 detection on gastric cancer tissues. Further, the hyaluronan-binding domain of CD44 was docked with RP-1 using computer modeling/docking approaches, revealing a RP-1/CD44 interaction with geometrical and energy match (-8.6 kcal/mol).
Conclusions:
The RP-1 peptide we screened exhibits affinity and specificity to CD44 on cells and has the potential to be used as a candidate probe for gastric cancer cell targeting.
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