Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies
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Updated: Apr 7, 2026

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Published on: May 27, 2019
Isaac Vizcaino-Caston1, Catherine A Kelly2, Annabel V L Fitzgerald2
1School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK; Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Abstract:
We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using Fourier transform infrared spectroscopy and differential scanning calorimetry.
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