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Related Concept Videos

Bioplastics01:27

Bioplastics

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Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
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Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
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Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies.

Isaac Vizcaino-Caston1, Catherine A Kelly2, Annabel V L Fitzgerald2

  • 1School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK; Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.

Journal of Bioscience and Bioengineering
|July 6, 2015
PubMed
Summary

A new method using dimethyl sulphoxide (DMSO) efficiently extracts Polyhydroxyalkanoate (PHA) from Cupriavidus necator cells. This novel PHA extraction technique yields comparable results to traditional chloroform methods for screening studies.

Keywords:
BioplasticDimethyl sulphoxidePolyhydroxyalkanoateSolvent extraction

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Area of Science:

  • Biotechnology
  • Polymer Science
  • Microbiology

Background:

  • Polyhydroxyalkanoates (PHAs) are biodegradable polymers with significant industrial potential.
  • Efficient extraction methods are crucial for PHA characterization and application development.
  • Conventional extraction methods, like those using chloroform, pose environmental and safety concerns.

Purpose of the Study:

  • To introduce and evaluate a novel Polyhydroxyalkanoate (PHA) extraction method using dimethyl sulphoxide (DMSO).
  • To compare the efficacy of DMSO-based PHA extraction with conventional chloroform extraction.
  • To assess the properties of PHA extracted via the DMSO method for screening applications.

Main Methods:

  • Extraction of Polyhydroxyalkanoate (PHA) from Cupriavidus necator cells using dimethyl sulphoxide (DMSO).
  • Comparative analysis of PHA yield and properties against conventional chloroform extraction.
  • Characterization of extracted PHA using Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).

Main Results:

  • The DMSO extraction method released comparable quantities of PHA from Cupriavidus necator cells.
  • PHA extracted using DMSO exhibited properties similar to those obtained via chloroform extraction.
  • Fourier transform infrared spectroscopy and differential scanning calorimetry confirmed the comparable properties of the extracted PHA.

Conclusions:

  • Dimethyl sulphoxide (DMSO) offers a viable and effective alternative for Polyhydroxyalkanoate (PHA) extraction in screening studies.
  • The DMSO method provides comparable PHA yields and properties to chloroform extraction, with potential advantages in safety or environmental impact.
  • This novel extraction technique supports efficient screening and characterization of PHAs for biotechnological applications.