Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors01:24

Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors

1.6K
Peptic ulcer disease, commonly called PUD, represents a multifaceted condition characterized by disruptions in the lining of the gastrointestinal (GI)  tract. Central to the protection of the gastrointestinal lining is the mucosal-bicarbonate barrier. This physiological defense mechanism is a formidable shield against the corrosive effects of gastric acid and pepsin secretion in the stomach. Its role is pivotal in maintaining the structural integrity of the stomach's inner lining.
1.6K
Drugs for Treatment of Ulcerative Colitis in IBD01:29

Drugs for Treatment of Ulcerative Colitis in IBD

654
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
654
Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists01:23

Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists

1.3K
Serotonin, a crucial neurotransmitter synthesized by enterochromaffin cells, plays a cardinal role in regulating gastrointestinal (GI) motility. With over 90% of the body's total serotonin in the GI tract, its influence on digestive processes is profound. Serotonin is swiftly released upon various stimuli, such as food boluses or certain drugs, triggering intrinsic sensory neurons in the myenteric plexus and extrinsic vagal and spinal sensory neurons. This leads to the activation of the...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activation of epidermal melanocytes in hidradenitis suppurativa.

The Journal of investigative dermatology·2026
Same author

Surgical innovation and technology.

The British journal of surgery·2026
Same author

Coordinated regulation of colonic fluid and mucus secretion.

Frontiers in pharmacology·2026
Same author

Outcomes from the English National Lynch Syndrome transformation project.

International journal of cancer·2026
Same author

Global benchmarks for minimally invasive right hemicolectomy for cancer.

The British journal of surgery·2025
Same author

Appraisal of the robotic approach to beyond-TME via video-based materials; a systematic review and evaluation utilising the IDEAL framework.

Journal of robotic surgery·2025

Related Experiment Video

Updated: Apr 7, 2026

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
06:31

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

1.7K

The effects of polyamines on human colonic mucosal function.

Ailín C Rogers1, Frank D McDermott1, Helen M Mohan1

  • 1Department of Surgery, St Vincent's University Hospital, Elm Park, Dublin 4, Ireland; School of Veterinary Medicine and Conway Institute of Biomolecular & Biomedical Science, University College Dublin, Dublin 4, Ireland.

European Journal of Pharmacology
|July 7, 2015
PubMed
Summary

Polyamines in the colon inhibit cyclic AMP-mediated chloride secretion by reducing intracellular cAMP levels. They also modulate calcium signaling, impacting colonic mucosal function.

Keywords:
Calcium-sensing receptorColonPolyaminesUssing chambers

More Related Videos

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
08:58

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

13.0K
Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer
06:24

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

1.8K

Related Experiment Videos

Last Updated: Apr 7, 2026

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
06:31

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

1.7K
Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
08:58

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

13.0K
Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer
06:24

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

1.8K

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Cell Physiology

Background:

  • Electrogenic ion transport in the human colon reflects mucosal function and is influenced by various mediators.
  • Polyamines are abundant in the colon and crucial for cellular processes.

Purpose of the Study:

  • To investigate polyamine mechanisms on colonic tissue.
  • To examine polyamine effects on secretory pathways, intracellular cAMP, and Ca(2+) accumulation.

Main Methods:

  • Human colonic mucosa mounted in Ussing chambers to record ion transport changes upon polyamine treatment.
  • Colonic crypts treated with polyamines for intracellular cAMP determination (ELISA) and Ca(2+) quantification (fluorescent imaging).

Main Results:

  • Physiological polyamine concentrations (1mM) did not affect basal chloride secretion or electrical resistance.
  • Polyamines inhibited forskolin-stimulated (cAMP-mediated) but not carbachol-stimulated (Ach-mediated) ion secretion.
  • All tested polyamines (spermine, spermidine, putrescine) reduced intracellular cAMP accumulation.
  • Spermine elevated intracellular Ca(2+) via a PKC-dependent pathway, potentially involving the CaSR.
  • Polyamines prevent cAMP-mediated chloride hypersecretion by inhibiting PKC-mediated intracellular calcium release.

Conclusions:

  • Polyamines regulate colonic ion transport by modulating cAMP and calcium signaling pathways.
  • These findings highlight a novel mechanism for polyamine action in the human colon, impacting mucosal function.