Development of a fabric phase sorptive extraction with high-performance liquid chromatography and ultraviolet...

A novel analytical method has been developed and validated for the quantification of alkyl phenols in aqueous and soil samples. Fabric phase sorptive extraction, a new sorptive microextraction technique, has been employed for the preconcentration of some endocrine-disruptor alkylphenol molecules, namely, 4-tert-butylphenol, 4-sec-butylphenol, 4-tert-amylphenol, and 4-cumylphenol, followed by high-performance liquid chromatography with ultraviolet detection. Various parameters influencing the fabric phase sorptive extraction performance, namely, extraction time, eluting solvent, elution time and pH of the sample matrix, were optimized. The chromatographic separation was carried out with a mobile phase of acetonitrile/water (60:40 v/v) at an isocratic flow rate of 1.0 mL/min using a reversed-phase C₁₈ column at λ_max 225 nm. The calibration curves of target analytes were prepared in the concentration range 5-500 ng/mL with good coefficient of determination values (R² > 0.992). Extraction efficiency values were 74.0, 75.6, 78.0, and 78.3 for 4-tert-butylphenol, 4-sec-butylphenol, 4-tert-amylphenol, and 4-cumylphenol, respectively. The limits of detection range from 0.161 to 0.192 ng/mL. Subsequently, the new fabric phase sorptive extraction with high-performance liquid chromatography and ultraviolet detection was successfully applied for the recovery of alkyl phenols from spiked ground water, river water, and treated water from a sewage treatment plant, and soil and sludge samples.