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Updated: Apr 7, 2026

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
Published on: September 23, 2020
Anagliptin, a potent dipeptidyl peptidase IV inhibitor: its single-crystal structure and enzyme interactions
Yukihisa S Watanabe1, Yoshika Yasuda1, Yuko Kojima2
1a Mie Research Park, Sanwa Kagaku Kenkyusho Co., Ltd. , Mie , Japan .
Abstract:
The single-crystal structure of anagliptin, N-[2-({2-[(2S)-2-cyanopyrrolidin-1-yl]-2-oxoethyl}amino)-2-methylpropyl]-2-methylpyrazolo[1,5-a]pyrimidine-6-carboxamide, was determined. Two independent molecules were held together by intermolecular hydrogen bonds, and the absolute configuration of the 2-cyanopyrrolidine ring delivered from l-prolinamide was confirmed to be S. The interactions of anagliptin with DPP-4 were clarified by the co-crystal structure solved at 2.85 Å resolution. Based on the structure determined by X-ray crystallography, the potency and selectivity of anagliptin were discussed, and an SAR study using anagliptin derivatives was performed.
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