Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Labeling DNA Probes03:31

Labeling DNA Probes

9.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Publisher Correction: F- and G-actin homeostasis regulates mechanosensitive actin nucleation by formins.

Nature cell biology·2026
Same author

Particulate thiols along a meridional transect in the western North Pacific: Insights from laboratory cultures of Synechococcus sp. and Thalassiosira nordenskioeldii.

The Science of the total environment·2025
Same author

Phenylalanine amide derivatives promote FLG expression via AHR activation in normal human epidermal keratinocytes.

Journal of pharmacological sciences·2025
Same author

Evaluation of dipole moment of polyhedral oligomeric silsesquioxane compounds.

Dalton transactions (Cambridge, England : 2003)·2024
Same author

Geometry Optimization Using the Frozen Domain and Partial Dimer Approaches in the Fragment Molecular Orbital Method: Implementation, Benchmark, and Applications to Protein Ligand-Binding Sites.

Journal of chemical information and modeling·2024
Same author

Performance of deep learning models in predicting the nugent score to diagnose bacterial vaginosis.

Microbiology spectrum·2024

Related Experiment Video

Updated: Apr 7, 2026

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.9K

Multitarget super-resolution microscopy with high-density labeling by exchangeable probes.

Tai Kiuchi1, Makio Higuchi1, Akihiro Takamura2

  • 1Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto, Japan.

Nature Methods
|July 7, 2015
PubMed
Summary
This summary is machine-generated.

We developed image reconstruction by integrating exchangeable single-molecule localization (IRIS), a super-resolution microscopy method. IRIS achieves high labeling density for visualizing molecular proximity in cells.

More Related Videos

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
07:44

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

4.6K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.2K

Related Experiment Videos

Last Updated: Apr 7, 2026

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.9K
Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
07:44

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

4.6K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.2K

Area of Science:

  • Cellular biology
  • Microscopy
  • Biophysics

Background:

  • Super-resolution microscopy is crucial for visualizing subcellular structures.
  • Current techniques face limitations in labeling density and multiplexing.
  • Understanding molecular interactions requires high-resolution imaging.

Purpose of the Study:

  • To develop a novel super-resolution microscopy technique for high-density, multi-target imaging.
  • To enable precise visualization of molecular proximity within cellular structures.
  • To overcome limitations of existing single-molecule localization microscopy methods.

Main Methods:

  • Developed image reconstruction by integrating exchangeable single-molecule localization (IRIS).
  • Utilized protein fragment-based probes with millisecond association/dissociation kinetics.
  • Integrated single-molecule localization with sequential labeling strategies.

Main Results:

  • Achieved unprecedented labeling density along multiple cellular structures.
  • Demonstrated the ability to discern area-specific proximity between cytoskeletal components and focal adhesions.
  • Successfully visualized molecular interactions at high resolution within a single cell.

Conclusions:

  • IRIS is a powerful multitarget super-resolution microscopy technique.
  • IRIS enables detailed mapping of molecular proximities in complex cellular environments.
  • This method advances the study of cellular organization and function.