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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Tandem Mass Spectrometry01:21

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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High-Resolution Mass Spectrometry (HRMS)01:15

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Related Experiment Video

Updated: Apr 7, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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High-speed MALDI MS/MS imaging mass spectrometry using continuous raster sampling.

Boone M Prentice1,2, Chad W Chumbley3,2, Richard M Caprioli1,3,4,2

  • 1Department of Biochemistry, Vanderbilt University, Nashville, TN, 37232.

Journal of Mass Spectrometry : JMS
|July 8, 2015
PubMed
Summary
This summary is machine-generated.

High-speed mass spectrometry imaging using matrix-assisted laser desorption/ionization time of flight/time of flight tandem mass spectrometry (MALDI TOF/TOF) enables rapid analysis of tissues. This advanced technique significantly increases throughput for complex biological samples.

Keywords:
MALDIMS/MSTOF/TOFcontinuous rasterdrug imagingimaginglipid imaging

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Mass Spectrometry

Background:

  • Matrix-assisted laser desorption/ionization time of flight/time of flight tandem mass spectrometry (MALDI TOF/TOF) is crucial for molecular imaging.
  • Existing MS/MS instrumentation faces limitations in high-throughput analysis of complex biological tissues.

Purpose of the Study:

  • To develop and validate rapid MS/MS ion image acquisition methodologies using continuous laser raster sampling.
  • To enhance throughput and maintain structural identity confirmation in MALDI TOF/TOF imaging.

Main Methods:

  • Utilized a MALDI TOF/TOF mass spectrometer with a 5 kHz Nd:YLF laser and high digitizer acquisition rates.
  • Implemented continuous laser raster sampling for MS/MS experiments, including precursor/fragment ion transitions.
  • Analyzed thin tissue sections for the drug rifampicin and a kidney lipid (SM4s).

Main Results:

  • Achieved 8 to 14-fold increases in throughput compared to existing MS/MS instrumentation.
  • Demonstrated rapid MS/MS ion image acquisition from thin tissue sections.
  • Confirmed structural identity of analytes within the imaging experiment.

Conclusions:

  • Continuous laser raster sampling in MALDI TOF/TOF MS/MS imaging significantly boosts analytical throughput.
  • This methodology is advantageous for interrogating complex chemical environments like biological tissues.
  • The enhanced speed and specificity are critical for imaging large tissue areas.