Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

SDS-PAGE01:27

SDS-PAGE

36.2K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
36.2K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.8K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering of the hepatitis C virus helicase for enhanced seroreactivity.

Journal of virological methods·2018
Same author

Development of a dot-blot assay for screening monoclonal antibodies to low-molecular-mass drugs.

Analytical biochemistry·2010
Same author

Development of an Abbott ARCHITECT cyclosporine immunoassay without metabolite cross-reactivity.

Clinical biochemistry·2010
See all related articles

Related Experiment Video

Updated: Apr 7, 2026

Extraction and Characterization of Surfactants from Atmospheric Aerosols
09:34

Extraction and Characterization of Surfactants from Atmospheric Aerosols

Published on: April 21, 2017

17.4K

A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration.

Kevin R Rupprecht1, Ewa Z Lang1, Svetoslava D Gregory1

  • 1Diagnostics Analytical Chemistry Research, Abbott Laboratories, Abbott Park, IL 60049, USA.

Analytical Biochemistry
|July 8, 2015
PubMed
Summary

A new, precise method accurately quantifies sodium dodecyl sulfate (SDS) concentration in protein solutions. This improved technique enhances reproducibility for critical protein studies using SDS.

Keywords:
ConcentrationQuantitative analysisSodium dodecyl sulfate (SDS)

More Related Videos

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

3.8K
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

15.8K

Related Experiment Videos

Last Updated: Apr 7, 2026

Extraction and Characterization of Surfactants from Atmospheric Aerosols
09:34

Extraction and Characterization of Surfactants from Atmospheric Aerosols

Published on: April 21, 2017

17.4K
Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

3.8K
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

15.8K

Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Sodium dodecyl sulfate (SDS) is crucial for denaturing and solubilizing proteins, particularly hydrophobic ones.
  • Existing quantitative methods for SDS determination using Stains-All lack sufficient accuracy and reproducibility.
  • Accurate SDS quantification is vital for experiments involving protein solutions.

Purpose of the Study:

  • To develop a more accurate and reproducible quantitative method for determining sodium dodecyl sulfate (SDS) concentration.
  • To refine the existing Stains-All dye-based method for SDS quantification.

Main Methods:

  • Modified the Stains-All dye-based method for SDS quantification.
  • Investigated and optimized parameters including solvent, pH, buffers, and light exposure.
  • Focused on improving accuracy and precision for protein solutions.

Main Results:

  • Developed an improved method for quantifying SDS concentration.
  • The enhanced method demonstrates simplicity, robustness, accuracy, and high precision.
  • The modified procedure addresses the limitations of previous quantitative SDS assays.

Conclusions:

  • The refined Stains-All method provides a simple, robust, accurate, and precise way to quantify SDS.
  • This improved method is suitable for applications where precise SDS concentration is critical.
  • Enhanced reproducibility in SDS quantification supports more reliable protein research.