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Related Experiment Video

Updated: Apr 7, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Improved internal control for molecular diagnosis assays.

T Vinayagamoorthy1, Danielle Maryanski1, Dilanthi Vinayagamoorthy1

  • 1MultiGEN Diagnostics LLC, 854 Pargon Way, Rock Hill, SC 29730, United States.

Methodsx
|July 8, 2015
PubMed
Summary

The IC-Code platform offers a true internal control for molecular diagnostics, ensuring accurate DNA amplification and identification by using identical annealing sequences for both the control and analyte. This reduces false negatives and positives, improving diagnostic reliability.

Keywords:
AnalyteIC-CodeMultiplex PCRNucleic acid amplification testPb2aSanger sequencingTrue negative control for PCR amplification and DNA sequencing coupled analysis

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Molecular diagnostics rely on DNA amplification and identification.
  • Current amplification controls like housekeeping genes lack true internal control due to non-identical annealing conditions.
  • This can lead to inaccurate results, including false negatives.

Purpose of the Study:

  • To introduce the IC-Code platform technology for enhanced molecular diagnostic accuracy.
  • To present a novel internal control system that overcomes limitations of traditional amplification controls.
  • To improve the reliability of molecular diagnostic assays.

Main Methods:

  • The IC-Code platform utilizes identical PCR primer annealing sequences for both the internal control and the analyte.
  • It employs identical sequencing primer annealing sequences for the identification step.
  • DNA sequencing analysis is used for precise analyte identification.

Main Results:

  • The IC-Code platform ensures identical annealing conditions for the internal control and analyte.
  • This design significantly minimizes false negatives and false positives.
  • The lower limit of detection can be precisely set by adjusting the internal control concentration.

Conclusions:

  • The IC-Code platform provides a true internal control for molecular diagnostics.
  • This technology enhances the accuracy and reliability of DNA amplification and identification.
  • It offers a robust solution for reducing diagnostic errors.