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Improved method for enumerating sulfate-reducing bacteria using optical density.

L A Bernardez1, L R P de Andrade Lima2

  • 1Graduate Program of Industrial Engineering, Federal University of Bahia, Brazil.

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|July 8, 2015
PubMed
Summary
This summary is machine-generated.

This study presents a modified method for accurately measuring bacterial concentration in sulfate-reducing bacteria (SRB) cultures by acidifying samples. This technique overcomes interferences from precipitates, improving optical density measurements for bacterial dry mass estimation.

Keywords:
Iron sulfideOptical densityReducing interference in the analysis of the sulfate-reducing bacteria solution content by optical density using acidified solutionsSouringSulfate-reducing bacteria

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Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Environmental Science

Background:

  • Photometric determination of bacterial concentration is often hindered by secondary scattering and precipitate interferences.
  • Conventional methods for sulfate-reducing bacteria (SRB) use growth media with iron, leading to precipitates that interfere with optical density measurements.
  • Iron hydroxide precipitation at high pH values significantly affects cell population estimates.

Purpose of the Study:

  • To develop a modified method for accurate optical density measurement of SRB.
  • To overcome interferences caused by precipitates in bacterial concentration determination.
  • To improve the reliability of photometric methods for quantifying SRB in oil field produced water.

Main Methods:

  • Acidification of SRB mixed cultures from oil field produced water to a pH below 2 using hydrochloric acid.
  • Measurement of optical density to determine bacterial concentration.
  • Analysis of the relationship between bacterial dry mass and absorbance after acidification.

Main Results:

  • A modified method significantly improves optical density measurements for SRB.
  • The relationship between bacterial dry mass and absorbance in acidified solutions is exponential.
  • Acidification to pH below 2 does not compromise the integrity of SRB cells.

Conclusions:

  • The developed acidification method enhances the accuracy of photometric bacterial concentration determination.
  • This technique effectively mitigates interferences from iron sulfide and iron hydroxide precipitates.
  • The modified method is applicable for kinetic experiments and bacterial growth time evolution studies.