Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hierarchical interplay between H3K27ac and H3K4me3 in transcriptional regulation.

Nature communications·2026
Same author

Multi-omics and machine learning-based exploration of key genes associated with abdominal aortic aneurysm.

Frontiers in molecular biosciences·2026
Same author

Hierarchical Interplay between H3K27ac and H3K4me3 in Transcriptional Regulation.

bioRxiv : the preprint server for biology·2026
Same author

Hederagenin suppresses inflammation-cancer transformation in chronic atrophic gastritis by modulating glycolysis through the mTOR/HIF-1α axis.

European journal of pharmacology·2026
Same author

Cyclic γ-AApeptide-Based Molecular Glues for RNA m<sup>6</sup>A Editing.

ACS chemical biology·2026
Same author

Fabrication of SilMA hydrogels with stiffness gradients for soft-to-hard interface tissue engineering.

Colloids and surfaces. B, Biointerfaces·2026

Related Experiment Video

Updated: Apr 7, 2026

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

3.0K

3D Scaffolds with Different Stiffness but the Same Microstructure for Bone Tissue Engineering.

Guobao Chen1, Chanjuan Dong1, Li Yang1

  • 1†Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, and ‡'111' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing 400044, P. R. China.

ACS Applied Materials & Interfaces
|July 8, 2015
PubMed
Summary

This study developed novel 3D scaffolds with tunable stiffness but consistent microstructure to study stem cell differentiation. These scaffolds enhance osteogenic differentiation, cell recruitment, and angiogenesis in bone tissue engineering.

Keywords:
3D microenvironmentbone tissue engineeringdecellularized cancellous bonematrix stiffnessmesenchymal stem cellsosteogenic differentiation

More Related Videos

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.5K
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.2K

Related Experiment Videos

Last Updated: Apr 7, 2026

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

3.0K
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.5K
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.2K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Stem Cell Biology

Background:

  • Extracellular matrix (ECM) stiffness significantly influences stem cell behavior, including differentiation.
  • Existing 3D scaffold fabrication methods often alter structural properties when tuning stiffness, limiting in vivo relevance.
  • Stem cells' response to mechanical cues in a physiologically accurate 3D environment is crucial for regenerative medicine.

Purpose of the Study:

  • To develop novel 3D scaffolds with varying stiffness while maintaining a consistent 3D microstructure.
  • To evaluate the impact of tunable scaffold stiffness on mesenchymal stem cell (MSC) behavior in vitro and in vivo.
  • To assess the potential of these scaffolds for bone tissue engineering applications.

Main Methods:

  • Decellularized cancellous bone was used as a base for 3D scaffolds.
  • Collagen and hydroxyapatite mixtures were coated onto scaffolds to achieve distinct stiffness levels (local and bulk).
  • Microcomputed tomography (μ-CT) confirmed scaffold architectural integrity post-coating. Cell viability, osteogenic differentiation, cell recruitment, and angiogenesis were assessed.

Main Results:

  • Scaffolds exhibited tunable stiffness (e.g., local: 13-37 kPa, bulk: 6-23 kPa) without significant changes in 3D microstructure.
  • In vitro studies showed enhanced MSC adhesion, proliferation, and osteogenic differentiation on the developed scaffolds.
  • In vivo experiments demonstrated successful MSC recruitment, osteogenic differentiation, and angiogenesis within the scaffolds.

Conclusions:

  • A novel method for creating 3D scaffolds with tunable mechanical properties and preserved microstructure was successfully developed.
  • These scaffolds effectively promote osteogenic differentiation, MSC recruitment, and angiogenesis, showing promise for bone regeneration.
  • The findings highlight the importance of matrix stiffness in 3D stem cell differentiation and have broad implications for tissue engineering and regenerative medicine.