Split-inteins and their bioapplications
Related Experiment Video
Updated: Apr 7, 2026

Synthesis of an Intein-mediated Artificial Protein Hydrogel
Published on: January 27, 2014
1R & D Services, Cytovance Biologics 800 Research Parkway, Suite 200, Oklahoma City, OK, 73104, USA. yifgli@gmail.com.
Abstract:
Split-inteins are a subset of inteins that are expressed in two separate halves and catalyze splicing in trans upon association of the two domains. They occur naturally and have also been artificially generated by splitting of contiguous ones. With their unique properties, split-inteins offer improved controllability, flexibility and capability to existing tools based on contiguous inteins. In addition, split-inteins have proven useful in several new applications. This review gives a general introduction to split-inteins with a focus on their role in expanding the applications of intein-based technologies.
More Related Videos
Related Concept Videos
Tagging and Fusion Proteins
Production of Pharmaceuticals
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Non-LTR Retrotransposons
RNA Splicing
Integrins
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...

