Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Foundation model enables interpretable open and error-tolerant searching for mass spectrometry-based proteomics.

Bioinformatics (Oxford, England)·2026
Same author

Newborns with sickle cell trait have distinct peripheral blood molecular signatures across the first week of life.

Blood. Red cells & iron·2026
Same author

Super-Resolution 3D Imaging Reveals Disarray of Dyadic Calcium Ion Channels in Failing Hearts Expressing Low Thyroid Hormone Function.

International journal of molecular sciences·2026
Same author

MYC-Mediated USP39 Upregulation Stabilizes SRSF1 in Pancreatic Cancer.

Molecular cancer research : MCR·2026
Same author

Co-option of lineage plasticity as a hallmark of multipotent acute leukemias.

Blood neoplasia·2026
Same author

Early immune events during SARS-CoV-2 infection impact memory T and B cell responses.

Communications biology·2026

Related Experiment Video

Updated: Apr 7, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K

A High-Efficiency Cellular Extraction System for Biological Proteomics.

Avantika Dhabaria, Paolo Cifani, Casie Reed

  • 1‡Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, United States.

Journal of Proteome Research
|July 9, 2015
PubMed
Summary
This summary is machine-generated.

This study optimized mechanical cell lysis for protein extraction, finding adaptive focused acoustic (AFA) sonication with a specific detergent mix best preserves native protein activity for proteomics. This method enhances proteome depth and yield for biochemical analyses.

Keywords:
adaptive focused acousticsbinary detergentcell lysisprotein extraction

More Related Videos

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

4.6K
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
11:12

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

12.9K

Related Experiment Videos

Last Updated: Apr 7, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K
Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

4.6K
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
11:12

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

12.9K

Area of Science:

  • Biochemistry
  • Proteomics
  • Cell Biology

Background:

  • Quantitative high-resolution mass spectrometry requires sensitive and specific proteome extraction methods.
  • Preserving native protein activity during cell lysis is crucial for accurate biochemical analyses.

Purpose of the Study:

  • To systematically analyze mechanical cell lysis and protein extraction methods.
  • To identify methods that maximize protein yield and depth while minimizing denaturation.

Main Methods:

  • Mechanical disruption using Potter-Elvehjem homogenization and acoustic sonication (probe and AFA).
  • Evaluation of various detergents (polyoxyethylene ethers/esters, glycosides, zwitterions).
  • Analysis using fluorescence spectroscopy, biochemical assays, and mass spectrometry proteomics.

Main Results:

  • Adaptive focused acoustic (AFA) sonication with a binary poloxamer mixture (octyl-β-glucoside and Pluronic F-127) maximized proteome extraction depth and yield.
  • This method maintained native protein activity, comparable to denaturing buffers like SDS.
  • Efficient extraction of major cellular organelles was achieved.

Conclusions:

  • AFA sonication combined with a specific binary poloxamer detergent system provides high-efficiency native proteome extraction.
  • This optimized method is suitable for various cell biochemical studies, including structural and functional proteomics.
  • The findings advance sample preparation techniques for mass spectrometry-based analyses.