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Related Concept Videos

Redox Reactions01:24

Redox Reactions

59.4K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
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Redox Titration: Overview01:21

Redox Titration: Overview

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Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...
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Redox Titration: Other Oxidizing and Reducing Agents01:26

Redox Titration: Other Oxidizing and Reducing Agents

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Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...
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Redox Titration: Iodimetry and Iodometry01:23

Redox Titration: Iodimetry and Iodometry

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Iodometry and iodimetry are analytical methods used to determine the concentration of oxidizing or reducing agents using iodine. In iodometric titrations, the oxidizing analyte solution is usually acidified and treated with an excess of iodide ions, which generates an equivalent amount of iodine in equilibrium with triiodide. The released iodine is subsequently titrated directly against a standardized reducing agent. As the dilute iodine color becomes pale yellow, a few drops of freshly...
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Related Experiment Video

Updated: Apr 7, 2026

Cellular Redox Profiling Using High-content Microscopy
11:37

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

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Quantitative Redox Imaging Software.

Mark D Fricker1

  • 1Department of Plant Sciences, University of Oxford , Oxford, United Kingdom .

Antioxidants & Redox Signaling
|July 9, 2015
PubMed
Summary
This summary is machine-generated.

Spectral shift fluorescent probes offer reliable live-cell imaging for dynamic physiological processes. Software tools enable rapid analysis of complex multi-channel ratio imaging data from living cells.

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Area of Science:

  • Cellular and Molecular Imaging
  • Biophysics
  • Biochemistry

Background:

  • Ratiometric fluorescent reporters like reduction-oxidation-sensitive green fluorescent protein (roGFP) enable in vivo redox imaging.
  • roGFP can be targeted to specific cellular compartments for precise measurements.
  • Multi-channel imaging allows simultaneous monitoring of various physiological parameters.

Purpose of the Study:

  • To highlight the need for live cell imaging to capture transient physiological behaviors.
  • To introduce software for analyzing multi-channel ratio imaging data.
  • To discuss the advantages of spectral shift probes for quantitative measurements.

Main Methods:

  • Utilizing fluorescent reporters with spectral shifts for quantitative measurements.
  • Employing ratiometric measurements at two wavelengths.
  • Developing and applying software for multi-channel ratio imaging analysis.

Main Results:

  • Spectral shifts are independent of external factors like illumination intensity and probe concentration.
  • Ratiometric measurements provide reliable data for dynamic physiological processes.
  • Software facilitates intuitive analysis of large imaging datasets.

Conclusions:

  • Spectral shift probes are superior for quantitative live-cell imaging due to their robustness.
  • Advanced software is crucial for extracting meaningful data from complex imaging experiments.
  • Future work should focus on efficient data extraction and analysis from large imaging datasets.