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Related Concept Videos

Voltammetry: Stripping Methods01:13

Voltammetry: Stripping Methods

1.2K
Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
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Voltammetric Techniques: Pulse Voltammetry01:17

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Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
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Voltammetric Techniques: Linear-Scan (E vs Time)01:12

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1.5K
Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
1.5K
Voltammetry: Overview01:20

Voltammetry: Overview

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Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
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Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

2.0K
Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
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High-Performance Liquid Chromatography: Types of Detectors01:15

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass
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Absorptive stripping voltammetry for cannabis detection.

Rita Nissim1, Richard G Compton1

  • 1Department of Chemistry, Physical & Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford, OX1 3QZ UK.

Chemistry Central Journal
|July 9, 2015
PubMed
Summary
This summary is machine-generated.

Reliable detection of Δ(9)-tetrahydrocannabinol (THC), a cannabis constituent impairing driving, is crucial. An optimized carbon paste electrode using Absorptive Stripping Voltammetry offers sensitive THC detection in aqueous and synthetic saliva solutions.

Keywords:
Absorptive stripping voltammetryCannabis detectionCarbon paste electrodeLiquid-liquid interfacesTHC detectionΔ9-tetrahydrocannabinol

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Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Forensic Science

Background:

  • Δ(9)-tetrahydrocannabinol (THC) is the primary psychoactive component of cannabis.
  • THC significantly impairs driving ability, contributing to drug-driving accidents.
  • Reliable and sensitive detection methods for THC are essential for public safety.

Purpose of the Study:

  • To develop and optimize a sensitive electrochemical sensor for Δ(9)-tetrahydrocannabinol (THC) detection.
  • To evaluate the sensor's performance in aqueous solutions and synthetic saliva.
  • To establish practical and theoretical limits of detection for THC.

Main Methods:

  • Fabrication of an optimized carbon paste electrode using graphite powder and mineral oil.
  • Application of Absorptive Stripping Voltammetry for pre-concentration and detection of THC.
  • Testing the sensor in aqueous borate buffer solutions (pH 10.0) and synthetic saliva.

Main Results:

  • Sensitive detection of THC achieved in both aqueous and synthetic saliva solutions.
  • Practical limits of detection as low as 0.50 μM (stationary) and 0.10 μM (stirred) in buffer.
  • Theoretical limits of detection determined to be 0.48 nM (stationary) and 0.41 nM (stirred) in buffer.
  • Detection of THC at concentrations as low as 0.50 μM in synthetic saliva.

Conclusions:

  • Absorptive Stripping Voltammetry, with assay optimization, is a reliable method for THC detection.
  • The developed sensor achieves usefully low practical limits of detection.
  • The sensor shows potential for detecting THC in complex matrices like saliva.