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Sequence and expression of the murine diazepam binding inhibitor
G P Owens1, A K Sinha, J M Sikela
1Department of Cellular and Structural Biology, University of Colorado School of Medicine, Denver 80262.
Brain Research. Molecular Brain Research
|November 1, 1989
Summary
Diazepam binding inhibitor (DBI) is a brain protein that may modulate GABAA receptors. Mouse DBI cDNA was isolated, revealing conserved amino acid sequences across mammals and widespread mRNA distribution, suggesting broader functions beyond anxiety regulation.
Area of Science:
- Neuroscience
- Molecular Biology
- Biochemistry
Background:
- Diazepam binding inhibitor (DBI), also known as endozepine, is implicated in anxiety regulation.
- DBI is thought to modulate the gamma-aminobutyric acid receptor complex (GABAA).
Purpose of the Study:
- To isolate and characterize the cDNA clone for mouse diazepam binding inhibitor (DBI).
- To investigate the distribution and potential broader functions of DBI beyond its role in anxiety.
Main Methods:
- Screening of a mouse brain mRNA expression library using anti-DBI antisera.
- Isolation and sequencing of a DBI cDNA clone.
- Comparison of amino acid sequences across mammalian species.
- Hybridization analysis of DBI mRNA levels in various organs.
Main Results:
- A cDNA clone for mouse DBI was successfully isolated and sequenced.
- Mouse DBI consists of 87 amino acids, showing conserved size across human, rat, and bovine DBI.
- DBI mRNA (approximately 600 nucleotides) is present not only in the brain but also in organs like the liver and kidney.
- DBI mRNA exhibits moderate to high abundance in several organs.
Conclusions:
- The conserved structure of DBI across mammals suggests an important biological role.
- The widespread distribution of DBI mRNA indicates functions beyond the central nervous system and potential roles beyond modulating benzodiazepine binding sites.