Expression and significance of CHIP in canine mammary gland tumors
Huanan Wang1, Xu Yang, Yipeng Jin
1The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China.
Abstract:
CHIP (Carboxy terminus of Hsc70 Interacting Protein) is an E3 ubiquitin ligase that can induce ubiquitination and degradation of several oncogenic proteins. The expression of CHIP is frequently lower in human breast cancer than in normal breast tissue. However, the expression and role of CHIP in the canine mammary gland tumor (CMGT) remain unclear. We investigated the potential correlation between CHIP expression and mammary gland tumor prognosis in female dogs. CHIP expression was measured in 54 dogs by immunohistochemistry and real-time RT-PCR. CHIP protein expression was significantly correlated with the histopathological diagnosis, outcome of disease and tumor classification. The transcriptional level of CHIP was significantly higher in normal tissues (P=0.001) and benign tumors (P=0.009) than it in malignant tumors. CHIP protein expression was significantly correlated with the transcriptional level of CHIP (P=0.0102). The log-rank test survival curves indicated that patients with low expression of CHIP had shorter overall periods of survival than those with higher CHIP protein expression (P=0.050). Our data suggest that CHIP may play an important role in the formation and development of CMGTs and serve as a valuable prognostic marker and potential target for genetic therapy.
Insights
Carboxy terminus of Hsc70 Interacting Protein (CHIP) expression is lower in malignant canine mammary tumors. Lower CHIP protein levels correlate with poorer prognosis, suggesting CHIP as a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Veterinary Medicine
Background:
- Carboxy terminus of Hsc70 Interacting Protein (CHIP) is an E3 ubiquitin ligase.
- CHIP targets oncogenic proteins for degradation.
- CHIP expression is reduced in human breast cancer, but its role in canine mammary tumors is unknown.
Purpose of the Study:
- To investigate the expression and prognostic significance of CHIP in canine mammary gland tumors (CMGTs).
- To explore the correlation between CHIP expression and CMGT prognosis in female dogs.
Main Methods:
- Immunohistochemistry and real-time RT-PCR were used to measure CHIP expression in 54 dogs.
- Correlation analysis was performed between CHIP expression, histopathological diagnosis, and disease outcome.
- Survival analysis using log-rank test was conducted.
Main Results:
- CHIP expression significantly correlated with histopathological diagnosis, disease outcome, and tumor classification.
- Transcriptional levels of CHIP were significantly higher in normal and benign tissues compared to malignant tumors.
- Lower CHIP protein expression was associated with shorter overall survival periods in dogs with CMGTs.
Conclusions:
- CHIP plays a significant role in the development of CMGTs.
- CHIP is a potential prognostic marker for CMGTs.
- CHIP may be a viable target for canine mammary tumor genetic therapy.
