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Updated: Apr 7, 2026

Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
DNA Brushing Shoulders: Targeted Looping and Scanning of Large DNA Strands
Zubair Azad1, Maedeh Roushan1, Robert Riehn1
1NC State University, Raleigh, North Carolina 27695, United States.
Abstract:
We present a nanofluidic device for targeted manipulations in the quarternary structure of single DNA molecules. We demonstrate the folding and unfolding of hairpin-shaped regions, similar to chromatin loops. These loops are stable for minutes at nanochannel junctions. We demonstrate continuous scanning of two DNA segments that occupy a common nanovolume. We present a model governing the stability of loop folds and discuss how the system achieves specific DNA configurations without operator intervention.
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