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Interaction of type I collagen fibrils with phospholipid vesicles
A Martinez del Pozo1, M Onaderra, J Laynez
1Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain.
Abstract:
Type I collagen fibrils interact with phosphatidylcholine and phosphatidylglycerol vesicles. Fluorescence polarization of (1,6-diphenyl-1,3,5-hexatriene) DPH-labeled vesicles, circular dichroism and differential scanning calorimetry studies have been performed. The protein-lipid interaction produces a decrease of the enthalpy of the phospholipid phase transition. Positive charges of lysine residues of the protein are involved in the interaction as experiments with succinylated collagen show. The kinetic parameters and the extent of the fibrillogenesis of collagen are modified by the phospholipid vesicles.