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Multimodal reconstruction of microvascular-flow distributions using combined two-photon microscopy and Doppler
Louis Gagnon1, Sava Sakadžić2, Fréderic Lesage3
1Athinoula A. Martinos Center for Biomedical Imaging , Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States ; Harvard-MIT Division of Health Sciences and Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States ; Ecole Polytechnique de Montreal , Department of Electrical Engineering, 2900 Boulevard Edouard-Montpetit, Montreal, Quebec H3T 1J4, Canada.
Abstract:
Computing microvascular cerebral blood flow ([Formula: see text]) in real cortical angiograms is challenging. Here, we investigated whether the use of Doppler optical coherence tomography (DOCT) flow measurements in individual vessel segments can help in reconstructing [Formula: see text] across the entire vasculature of a truncated cortical angiogram. A [Formula: see text] computational framework integrating DOCT measurements is presented. Simulations performed on a synthetic angiogram showed that the addition of DOCT measurements, especially close to large inflowing or outflowing vessels, reduces the impact of pressure boundary conditions and estimated vessel resistances resulting in a more accurate reconstruction of [Formula: see text]. Our technique was then applied to reconstruct microvascular flow distributions in the mouse cortex down to [Formula: see text] by combining two-photon laser scanning microscopy angiography with DOCT.
