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Using simultaneous voltage and calcium imaging to study fast Ca(2+) channels.

Nadia Jaafari1, Elodie Marret1, Marco Canepari1

  • 1Inserm U836 , Grenoble Institute of Neuroscience, Team 3, Grenoble Cedex 09, France ; Université Joseph Fourier , Laboratoire Interdisciplinare de Physique (CNRS UMR 5588), F-38000 Grenoble, France ; Laboratories of Excellence , Ion Channel Science and Therapeutics, France.

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|July 10, 2015
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Summary

New optical methods allow precise correlation of cellular electrical and calcium activity. This technique enables the extraction of ion channel currents from imaging experiments, advancing voltage imaging capabilities.

Keywords:
calcium currentscalcium imagingvoltage imaging

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Area of Science:

  • Neuroscience
  • Biophysics
  • Cellular Electrophysiology

Background:

  • Voltage imaging has advanced significantly over 40 years.
  • Recent technical progress enables simultaneous measurement of membrane potential and intracellular calcium concentration.
  • This allows for spatially precise correlation of cellular electrical and calcium activity.

Purpose of the Study:

  • To demonstrate the use of advanced voltage imaging for investigating ion channel function.
  • To show how to extract ion currents from fluorescence imaging data.
  • To detail the methodology and application of simultaneous optical measurements.

Main Methods:

  • Utilizing fluorescence measurements of membrane potential and intracellular calcium.
  • Employing indicators that equilibrate in the intracellular space for accurate calcium measurement.
  • Describing a dedicated imaging apparatus and mathematical procedures for current extraction and signal calibration.

Main Results:

  • Demonstrated accurate derivation of ion channel kinetics from fluorescence measurements.
  • Successfully extracted ion currents from imaging experiments.
  • Presented simultaneous membrane potential and calcium optical measurements during an action potential in a hippocampal neuron.

Conclusions:

  • The combined optical approach offers a powerful tool for studying ion channel function.
  • This method allows for precise spatial and temporal correlation of electrical and calcium signaling.
  • The technique provides a detailed understanding of cellular electrophysiology and calcium dynamics.