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Updated: Feb 1, 2026

Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9
Published on: March 21, 2025
Construction of a CRISPR-Cas9 System for Pig Genome Targeting
Yu-Hsiu Su1, Tai-Yun Lin, Chung-Lin Huang
1a Division of Animal Technology, Laboratories of Animal Technology, Agricultural Technology Research Institute, Xiangshan District , Hsinchu City , Taiwan.
Abstract:
A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant protein in complex with sgRNA was translocated into the nucleus; the Flag2-NLS1-Cas9-NLS2 protein alone was excluded from the nucleus. Up to 13% of porcine PK1 cells targeted in vitro were observed, regardless of transfection efficiency.
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