Related Experiment Video

Updated: Apr 7, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.1K

Complete Genome Sequence of a CTX-M-15-Producing Klebsiella pneumoniae Outbreak Strain from Multilocus Sequence Type

Laura Becker1, Boyke Bunk, Christoph Eller1

  • 1Robert Koch Institute, FG13 Division of Nosocomial Pathogens and Antibiotic Resistances, Department of Infectious Diseases, Wernigerode Branch, Wernigerode, Germany.

Genome Announcements
|July 11, 2015
PubMed

Insights

We sequenced the genome of a multidrug-resistant Klebsiella pneumoniae strain responsible for a 2011 neonatal ward outbreak. This analysis provides insights into the genetic makeup of this significant public health threat.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Neonatal wards are vulnerable to hospital-acquired infections.
  • Multidrug-resistant (MDR) Klebsiella pneumoniae poses a significant threat to vulnerable populations.
  • Outbreaks of MDR pathogens necessitate rapid genomic investigation.

Purpose of the Study:

  • To perform whole-genome sequencing of a multidrug-resistant Klebsiella pneumoniae strain.
  • To characterize the genome of a strain that caused a neonatal ward outbreak in 2011.
  • To provide genomic data for understanding the spread and evolution of MDR Klebsiella pneumoniae.

Main Methods:

  • Whole-genome sequencing of the Klebsiella pneumoniae isolate.
  • Bioinformatic analysis of the resulting genome sequence.
  • Characterization of chromosomal and plasmid DNA.

Main Results:

  • The complete genome sequence of the multidrug-resistant Klebsiella pneumoniae strain was determined.
  • The genome comprises a single chromosome of 5,278 kb.
  • Three plasmids were identified with sizes of 362 kb, 5 kb, and 4 kb.

Conclusions:

  • The genome sequence provides a valuable resource for studying the genetic basis of multidrug resistance in Klebsiella pneumoniae.
  • Understanding the genomic structure aids in tracking and controlling outbreaks of this pathogen.
  • This data can inform future strategies for combating antimicrobial resistance in healthcare settings.

More Related Videos

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

6.0K
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

9.1K

Related Concept Videos

Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
48
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
840
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
480
Investigation of Disease Outbreaks01:23

Investigation of Disease Outbreaks

Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...
51