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Related Concept Videos

Methods of Nuclear Reprogramming01:24

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Dnmt3l-knockout donor cells improve somatic cell nuclear transfer reprogramming efficiency.

Hung-Fu Liao1, Chu-Fan Mo1, Shinn-Chih Wu1

  • 1Institute of BiotechnologyDepartment of Animal Science and TechnologyGenome and Systems Biology Degree ProgramNational Taiwan University, Taipei 106, TaiwanGenome and Systems Biology Degree ProgramAcademia Sinica, Taipei, TaiwanInstitut CurieCNRS UMR3244, Université Pierre et Marie Curie, 75248 Paris Cedex 05, FranceDepartment of Animal Science and BiotechnologyTunghai University, Taichung 407, TaiwanINSERM U934/CNRS UMR3215Institut Curie, 75005 Paris, FranceDepartment of Genetics and DevelopmentCollege of Physicians and Surgeons of Columbia University, New York, New York 10032, USAAgricultural Biotechnology Research CenterAcademia Sinica, Taipei 115, TaiwanCenter for Systems BiologyResearch Center for Developmental Biology and Regenerative MedicineNational Taiwan University, Taipei 106, Taiwan.

Reproduction (Cambridge, England)
|July 11, 2015
PubMed
Summary
This summary is machine-generated.

Using DNA methyltransferase-3-like knockout (Dnmt3l-KO) donor cells enhances cloned embryo development and quality. This epigenetic reprogramming improves developmental efficiency and blastocyst quality in nuclear transfer applications.

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Area of Science:

  • Developmental Biology
  • Epigenetics
  • Reproductive Technologies

Background:

  • Nuclear transfer (NT) is crucial for studying single-cell development and reprogramming.
  • DNA methyltransferase-3-like (Dnmt3l) acts as a transcriptional repressor and is expressed during early embryonic development.

Purpose of the Study:

  • To investigate the impact of Dnmt3l-knockout (Dnmt3l-KO) donor cells on cloned embryo development and quality.
  • To assess the epigenetic state of Dnmt3l-KO donor cells for improved nuclear transfer reprogramming.

Main Methods:

  • Nuclear transfer using Dnmt3l-knockout (Dnmt3l-KO) donor cells and Trichostatin A treatment.
  • Analysis of cloned embryo development, cell number, OCT4 expression, and transposable element silencing.
  • Epigenetic analysis of Dnmt3l-KO mouse embryonic fibroblasts, including histone mark profiling and HDAC1 localization.

Main Results:

  • Dnmt3l-KO donor cells significantly improved developmental efficiency and quality of cloned embryos.
  • Cloned embryos derived from Dnmt3l-KO cells showed increased cell numbers, restricted OCT4 expression in the inner cell mass (ICM), and silenced transposable elements.
  • Dnmt3l-KO donor cells exhibited a more permissive epigenetic state, with altered histone marks (increased H3K27ac, decreased H3K27me3/H3K9me3) and reduced HDAC1 nuclear localization.

Conclusions:

  • Dnmt3l-KO donor cells enhance nuclear transfer reprogramming, leading to improved cloned embryo development and quality.
  • The permissive epigenetic state of Dnmt3l-KO cells is beneficial for NT reprogramming, suggesting a role for Dnmt3l in regulating epigenetic modifications.
  • Zygotic Dnmt3l is not essential for pre-implantation cloned embryo development.