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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

18.6K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
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Related Experiment Video

Updated: Apr 7, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

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Hybridoma Technology.

Robert M Hnasko1, Larry H Stanker

  • 1Produce Safety and Microbiology Unit (PSM), Western Regional Research Center (WRRC), Pacific West Area (PWA), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 800 Buchanan St., Albany, CA, 94710, USA, Robert.hnasko@ars.usda.gov.

Methods in Molecular Biology (Clifton, N.J.)
|July 11, 2015
PubMed
Summary
This summary is machine-generated.

Hybridoma technology enables continuous, low-cost production of monoclonal antibodies (mAbs) by fusing immune cells with myeloma cells. This chapter details the essential materials and methods for generating mAb-producing hybridoma cell lines.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Hybridoma technology is a standard method for producing monoclonal antibodies (mAbs).
  • It involves fusing splenocytes from immunized animals with immortal myeloma cells.
  • Despite newer alternatives, hybridoma technology remains accessible and cost-effective for basic research.

Purpose of the Study:

  • To describe the materials and methodologies for generating monoclonal antibody-producing hybridoma cell lines.
  • To provide a guide for laboratories performing basic cell biological research.
  • To highlight the continued viability of hybridoma technology for antibody production.

Main Methods:

  • Fusion of splenocytes from immunized mice with immortal myeloma cells.
  • Culture and selection of hybridoma cell lines.
  • Screening for specific monoclonal antibody production.

Main Results:

  • Successful generation of hybridoma cell lines capable of producing specific monoclonal antibodies.
  • Demonstration of a viable and accessible method for antibody production.
  • Establishment of a continuous source of native whole immunoglobulins.

Conclusions:

  • Hybridoma technology is a robust and cost-effective method for producing monoclonal antibodies.
  • The described procedures facilitate the generation of mAb-producing hybridomas for various research applications.
  • This technique remains a valuable tool in cell biology and immunology research.