Related Experiment Video

Updated: Apr 7, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

826

New insights in molecular mechanisms involved in chronic kidney disease using high-resolution plasma proteome

Griet Glorieux1, William Mullen2, Flore Duranton3

  • 1Nephrology Section, Ghent University Hospital, Gent, Belgium.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|July 11, 2015
PubMed

Insights

Chronic kidney disease (CKD) alters plasma proteins, impacting health. This study identifies specific proteins, like leucine-rich alpha-2 glycoprotein, linked to vascular damage and mortality in CKD patients.

Area of Science:

  • Biochemistry
  • Nephrology
  • Proteomics

Background:

  • Reduced glomerular filtration rate in chronic kidney disease (CKD) causes accumulation of waste products.
  • CKD-associated morbidity and mortality may stem from these accumulated substances.
  • Previous research focused on individual proteins, necessitating a large-scale, integrated approach.

Purpose of the Study:

  • To comprehensively assess the plasma proteome in patients with varying stages of CKD.
  • To identify potential protein biomarkers associated with CKD progression and complications.
  • To explore the relationship between plasma protein changes and CKD-related outcomes.

Main Methods:

  • High-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyze plasma proteomes.
  • Discovery study included patients with stage 2-3 CKD (n=14) and stage 5 CKD on hemodialysis (HD) (n=15).
  • Enzyme-linked immunosorbent assay (ELISA) validated findings in a larger cohort (n=40).

Main Results:

  • 2054 proteins were detected; 127 showed decreased and 206 increased abundance in HD patients.
  • Pathway analysis revealed altered hemostasis, inflammation, complement activation, and vascular damage.
  • Lysozyme C and leucine-rich alpha-2 glycoprotein levels increased with CKD progression, linked to vascular damage and heart failure.

Conclusions:

  • This study offers a comprehensive view of the CKD plasma proteome.
  • Identified proteins may serve as novel biomarkers for CKD.
  • Further research is warranted to confirm the role of these proteins in CKD morbidity and mortality.
Abstract

Keywords:
biomarkerschronic kidney diseasehaemodialysismechanismsproteomicsuraemic solutes

More Related Videos

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes11:47

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

2.7K

Related Concept Videos

Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
1.2K
Chronic Kidney Disease III: Interprofessional Care01:28

Chronic Kidney Disease III: Interprofessional Care

Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
637
Chronic Kidney Disease II: Clinical Manifestations01:24

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
996
Acute Kidney Injury II: Pathophysiology01:29

Acute Kidney Injury II: Pathophysiology

Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
2.0K
Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
507
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
308