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Updated: Apr 7, 2026
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
Published on: December 8, 2023
Rapid detection of urinary long non-coding RNA urothelial carcinoma associated one using a PCR-free
Sanaa Eissa1, Marwa Matboli, Nada O E Essawy
1a Oncology Diagnostic Unit, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt .
Abstract:
We developed a specific hybridization assay for direct detection of long non-coding RNA urothelial carcinoma associated-1 (lncRNA-UCA1). Total RNA was extracted from urine pellet samples (bladder carcinoma patients and controls). Then, we compared the developed nanoassay with quantitative real time polymerase chain reaction (qRT-PCR) results in detection of urine UCA1 in bladder cancer and control samples. The sensitivity and the specificity of UCA1 nanoassay were 92.1% and 93.3%, respectively. The concordance of the two methods was 98%. Interestingly, all bilharzial benign cases showed negative lncRNA-UCA1 using both methods. UCA1-nanoassay is a valid test for direct detection of urine UCA1 for bladder cancer detection.
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