Related Experiment Video
Updated: Apr 7, 2026
Quasistatic Mechanical Testing for Computer-Aided Design and Manufacturing Occlusal Veneers Cemented to Milled Dentin Analog Material
Published on: December 20, 2024
Effect of method of caries induction on aged resin-dentin bond of primary teeth
Tathiane Larissa Lenzi1, Ana Flávia Bissoto Calvo2, Tamara Kerber Tedesco3
1Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, 05508-000, Brazil. tathilenzi@usp.br.
Background:
To investigate the influence of chemical and microbiological methods of caries induction on bond degradation of adhesive systems to primary dentin.
Methods:
Flat dentin surfaces from 36 primary molars were assigned to three groups (n = 12) according to method to induce caries-affected dentin: (1) control (sound dentin); (2) pH-cycling; and (3) microbiological caries induction model. Teeth were submitted to caries induction for 14 days for both methods, and the sound dentin was stored in distilled water during the same period. Specimens from each experimental group were then randomly reassigned to two subgroups (n = 6) according to the adhesive system tested: two-step etch-and-rinse adhesive (Adper Single Bond 2 - SB) or two-step self-etch system (Clearfil SE Bond - CSEB). Composite buildups were constructed and sectioned to obtain bonded sticks to be subjected to microtensile (μTBS) testing immediately or after 12 months of water aging. The μTBS means were analyzed by three-way repeated measures ANOVA and Tukey's tests (α = 0.05).
Results:
The μTBS values obtained to artificially-created caries-affected dentin were lower compared with sound dentin, but were not affected by method of caries induction. Water storage for 12 months reduced bond strengths, except to CSEB bonded to sound dentin.
Conclusion:
Chemical and microbiological methods affect similarly the stability of resin-dentin bonds in primary teeth.
