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Updated: Apr 7, 2026
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Feasible electrochemical biosensor based on plasma polymerization-assisted composite of polyacrylic acid and hollow
Zhihong Zhang1, Shuai Zhang2, Linghao He2
1Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, Zhengzhou, PR China; State Laboratory of Surface and Interface Science of Henan Province Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou 450001, PR China.
Abstract:
A composite made of polyacrylic acid and hollow TiO2 spheres (TiO2@PPAA) was prepared by the plasma polymerization method and subsequently used as an electrode material for detecting lysozyme. The chemical structure, surface morphology, and electrochemical performance of the TiO2@PPAA composite were mainly affected by the plasma input power used during plasma polymerization. After optimizing plasma conditions, aptamer strands exhibited high adsorption affinity toward the surface of TiO2@PPAA composite via synergistic effects between TiO2 and PPAA. Electrochemical impedance spectroscopy results showed that the developed TiO2@PPAA aptasensor presents highly sensitive detection ability toward lysozyme; the limit of detection of the proposed aptasensor is 0.015 ng mL(-1) (1.04 pM) within the range of 0.05-100 ng mL(-1) in terms of 3σ value. The film further showed excellent selectivity toward lysozyme in the presence of interfering proteins, such as thrombin, bovine serum albumin, and immunoglobulin E. Thus, this aptasensing strategy might broaden the applications of plasma polymerized nanomaterials in the field of biomedical research and early clinical diagnosis.
