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Proton pumping complex I increases growth yield in Candida utilis.

Nicole Avéret1, Marie-Lise Jobin1, Anne Devin1

  • 1Institute of Biochemistry and Genetics of the Cell, CNRS UMR 5095, 1 Rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France; Université de Bordeaux, 1 Rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France.

Biochimica Et Biophysica Acta
|July 13, 2015
PubMed
Summary

Candida utilis exhibits a 30% higher growth yield than Saccharomyces cerevisiae due to unique mitochondrial features. This enhanced efficiency in yeast growth is linked to proton-pumping complex I and external NADH dehydrogenase activity.

Keywords:
Candida utilisGrowth yieldMitochondriaOxidative phosphorylationSaccharomyces cerevisiaeYeast

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Area of Science:

  • Cellular metabolism
  • Microbial physiology
  • Biochemistry

Background:

  • Cellular growth depends on coupling catabolism with biomass formation and maintenance.
  • Growth yield, a measure of transformation efficiency, is critical for evaluating metabolic processes.
  • Candida utilis possesses a unique mitochondrial complex I and an external NADH dehydrogenase.

Purpose of the Study:

  • To investigate the higher growth yield in Candida utilis compared to Saccharomyces cerevisiae.
  • To elucidate the role of mitochondrial complex I and external NADH dehydrogenase in C. utilis growth.
  • To understand the regulation of electron transport and its impact on yeast growth yield.

Main Methods:

  • Comparative growth yield analysis between C. utilis and S. cerevisiae on non-fermentable media.
  • ADP/O determination to assess proton pumping efficiency of mitochondrial complexes.
  • Investigation of electron competition based on extra-mitochondrial NADH concentration.

Main Results:

  • C. utilis demonstrated a 30% higher growth yield than S. cerevisiae.
  • Electrons from internal NADH dehydrogenase utilize proton-pumping complex I, while external NADH dehydrogenase electrons do not.
  • Electron competition is regulated by extra-mitochondrial NADH levels, favoring biomass generation pathways.

Conclusions:

  • The unique mitochondrial respiratory chain in C. utilis enhances growth yield.
  • Regulation of electron entry into the respiratory chain optimizes energy production and biomass generation.
  • C. utilis exhibits a sophisticated metabolic strategy for efficient growth and NADH re-oxidation.