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Updated: Apr 7, 2026

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
Published on: February 7, 2017
Combining immunolabeling and catalyzed reporter deposition to detect intracellular saxitoxin in a cyanobacterium
Claudia Piccini1, Amelia Fabre2, Gissell Lacerot3
1Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Uruguay; Grupo Ecología y Fisiología de Fitoplancton, CSIC, Universidad de la República, Uruguay.
Abstract:
We combined the use of polyclonal antibodies against saxitoxin with catalyzed reporter deposition to detect production of saxitoxin by the cyanobacterium Cylindrospermopsis raciborskii. The procedure is simple, allows detection of intracellular saxitoxin in cyanobacteria filaments by confocal laser microscopy and is a promising tool to study toxin production and metabolism.
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