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Published on: November 2, 2009
A ratiometric fluorescent system for carboxylesterase detection with AIE dots as FRET donors
Yinglong Wu1, Shuailing Huang, Fang Zeng
1College of Materials Science and Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, P. R. China. mcfzeng@scut.edu.cn shzhwu@scut.edu.cn.
Abstract:
A ratiometric fluorescent system for CaE detection with AIE dots as the FRET donors was designed. Upon enzymatic reaction, electrostatic interaction between the cationic TPE-N(+) dots and the enzymatic reaction product - the negatively charged fluorescein molecules - allows the FRET process to proceed, thus affording the ratiometric fluorescence CaE assay.
