Related Experiment Video
Updated: Apr 7, 2026

08:32
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
13.4K
Nitroreductase-triggered activation of a novel caged fluorescent probe obtained from methylene blue
Jungeun Bae1, Louis E McNamara, Manal A Nael
1Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, MS 38677, USA. seongjo@olemiss.edu.
Abstract:
A near-infrared fluorescent probe based on methylene blue (p-NBMB) was developed for the detection of nitroreductase. Conjugating methylene blue with a p-nitrobenzyl moiety enables it to be activated by nitroreductase-catalyzed 1,6-elimination, resulting in the release of an active methylene blue fluorophore.

