Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differential analysis of allosteric behavior.

G H Czerlinski1

  • 1Northwestern University, Chicago, IL 60611.

Physiological Chemistry and Physics and Medical NMR
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resolving the fast equilibrations of ternary complexes in liver alcohol dehydrogenase.

Journal of theoretical biology·1993
Same author

Boundaries in gravitational and magnetic activation of cells for sorting.

Cell biophysics·1991
Same author

Cooperative coupling in allosteric systems.

Biophysical chemistry·1989
Same author

Coupling of redox indicator dyes into an enzymatic reaction cycle.

Journal of biochemical and biophysical methods·1988
Same author

Gravitational and magnetic activation methods applied to cultured cells (LK 35.2).

Journal of biochemical and biophysical methods·1987
Same author

Steady state enzyme kinetics for systems with three enzyme-binding species.

Physiological chemistry and physics and medical NMR·1986
Same journal

The physical state of potassium in frog skeletal muscle studied by ion-sensitive microelectrodes and by electron microscopy: interpretation of seemingly incompatible results.

Physiological chemistry and physics and medical NMR·2015
Same journal

Can we see living structure in a cell?

Physiological chemistry and physics and medical NMR·2015
Same journal

Preface for the updated Chinese translation of Gilbert N. Ling's Life at the Cell and Below-Cell Level.

Physiological chemistry and physics and medical NMR·2014
Same journal

A 2004 unanswered letter to the Economist magazine requesting a retraction (and apology).

Physiological chemistry and physics and medical NMR·2014
Same journal

What is life answered in terms of properties and activities of auto-cooperative assemblies of molecules, atoms, ions and electrons called nano-protoplasm.

Physiological chemistry and physics and medical NMR·2014
Same journal

Artifacts of electron microscopy in cells.

Physiological chemistry and physics and medical NMR·2011
See all related articles

Analyzing allosteric systems reveals subunit cooperativity using binding constant ratios. Calmodulin exhibits variable cooperativity based on experimental conditions, as predicted by the Monod model.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Allosteric systems involve complex molecular interactions.
  • Differential analysis of binding constants is crucial for understanding these systems.
  • The Adair and Monod models provide frameworks for studying subunit interactions.

Purpose of the Study:

  • To investigate cooperativity in allosteric systems using differential analysis of binding constants.
  • To evaluate the applicability of the Monod model to experimental data.
  • To analyze the binding behavior of calmodulin.

Main Methods:

  • Differential analysis of consecutive binding constants.
  • Comparison of experimental and statistical constant ratios.
  • Application of the Monod-Wyman-Changeux (MWC) model.

Related Experiment Videos

  • Analysis of calmodulin binding data.

    • Main Results:

    • A ratio of experimental to statistical constants smaller than 1 indicates cooperativity.
    • Calmodulin demonstrates context-dependent cooperativity among its subunits.
    • The Monod model effectively explains the observed binding patterns.
    • Estimation of the interconversion constant (Ln) is possible under specific conditions.

    Conclusions:

    • Differential analysis of binding constants is a robust method for detecting allosteric cooperativity.
    • Calmodulin's subunit interactions are complex and sensitive to experimental parameters.
    • The MWC model provides valuable insights into the allosteric regulation of calmodulin.