Effects of high-intensity treadmill training on timeliness and plasticity expression of irisin in mice

The purpose of this study was to investigate effects of high-intensity aerobic exercise training on timeliness and plasticity expression of irisin in mice and change of FNDC5, ACCβ expression, and to explore possible ways to influence its mechanism of fatty acid metabolism.

Adult male mice of specific pathogen-free grade [Kunming mice, (20 ± 2) g] are randomly divided into 4 groups. Wherein the first group is immediately after one-time exercise groups: including control group (CN group 1), 0.5 h exercise group (group 2), 1 h exercise group (group 3), 1.5 h exercise group (group 4) and 2 h exercise group (group 5), each for 10. The second group is rest after one-time 60 min exercise groups: including control group (CN group 1), rest 20 min groups (groups 2), rest 40 min group (group 3), rest 60 min group (groups 4), rest 80 min group (group 5), each for 10. Third group is immediately after long-term exercise groups: including the control group (CN group 1), 0.5 h exercise group (group 2), 1 h exercise group (group 3), 1.5 h exercise group (group 4) and 2 h exercise group (group 5), each for 10. The fourth group is rest after long-term 60 min exercise group: including control group (CN group 1), rest 20 min group (group 2), rest 40 min group (group 3), rest 60 min group (4 groups) and rest 80 min groups (5 groups), each for 10.

With the extension of a one-time high-intensity exercise time, the mouse FNDC5 protein, P-ACCβ / ACCβ ratio showed fluctuations, and opposite trends between the two, its turning points are 1.5 h; FNDC5 protein and P-ACCβ / ACCβ ratio with long-term exercise in mice at different time produce adaptability; the regulation of exercise induced irisin timeliness and plasticity reflected after a long-term exercise irisin expression in serum showed a steady decline in trend and return to normal levels, compared to a one-time exercise, expression of irisin is more stable.

With the high-intensity exercise a one-time extension of time, the mouse FNDC5 proteins, P-ACCβ / ACCβ ratio showed fluctuations, and both changes in the opposite trend, its turning points are 1.5 h; the long-term exercise can produce FNDC5 proteins, P-ACCβ / ACCβ ratios adaptable, more stable expression of the irisin curve after long-term exercise compared to a one-time exercise.