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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
Published on: September 10, 2013
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Novel stable dendrimersome formulation for safe bioimaging applications.
M Filippi1, D Patrucco, J Martinelli
1Dipartimento di Biotecnologie Molecolari e Scienze della Salute, Centro di Imaging Molecolare e Preclinico, Università degli Studi di Torino, Via Nizza 52, Torino, 10126, Italy. enzo.terreno@unito.it.
Nanoscale
|July 14, 2015
Summary
Newly developed dendrimersomes from Janus dendrimers show excellent stability and blood circulation times, comparable to liposomes. These nanovesicles are promising for advanced biomedical applications and drug delivery systems.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Dendrimersomes, formed by amphiphilic Janus dendrimers (JDs), are emerging nanocarriers for biomedical uses.
- Previous work demonstrated successful loading of contrast agents into dendrimersomes.
- Low-generation isomeric JDs offer potential for novel nanovesicle development.
Purpose of the Study:
- To synthesize and characterize novel low-generation Janus dendrimers (JDs).
- To evaluate the self-assembly and loading capabilities of these JDs into nanovesicles.
- To assess the in vitro stability and in vivo blood circulation of JD-based nanovesicles loaded with Gadoteridol.
Main Methods:
- Synthesis of two new isomeric JDs: JDG0G1(3,5) and JDG0G1(3,4).
- Self-assembly of JDs into vesicular nanoparticles.
- Loading of Gadoteridol (MRI contrast agent) into nanovesicles.
- In vitro stability tests in biological media.
- In vivo assessment of blood circulation lifetime in mice.
Main Results:
- Both JDs successfully formed almost monodisperse vesicular nanoparticles.
- Nanovesicles loaded with Gadoteridol exhibited promising stability in vitro.
- Nanovesicles derived from JDG0G1(3,5) showed spontaneous self-organization into homogeneous suspensions.
- In vivo studies indicated blood circulation lifetimes comparable to conventional liposomes.
- The nanovesicles demonstrated favorable safety and stability profiles.
Conclusions:
- Low-generation isomeric JDs can form stable, monodisperse nanovesicles suitable for biomedical applications.
- JDG0G1(3,5)-based nanovesicles offer a homogeneous suspension with excellent stability and circulation profiles.
- These novel dendrimersomes represent a promising platform for drug delivery and imaging contrast agent applications.
