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Updated: Apr 7, 2026
Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
A novel approach for quantitation of glucosylceramide in human dried blood spot using LC-MS/MS
Allena Ji Ji1, Haixing Wang1, Enida Ziso-Qejvanaj1
1Clinical Specialty Laboratory, DSAR, Sanofi, 1 The Mountain Road, Framingham, MA 01701, USA.
Background:
Glucosylceramide, an efficacy biomarker for Gaucher Type 1 disease, exhibits poor solubility in polar solvents and whole blood which makes it difficult to prepare a homogenous blood standard.
Results:
We developed a novel method using standard addition approach by spiking a small volume of analyte solution on the surface of prespotted dried blood spot. The whole spots were punched out for subsequent extraction and LC-MS/MS analysis. The assay performance met all validation acceptance criteria. Glucosylceramide concentrations in 50 paired plasma and dry blood spot samples obtained from Gaucher Type 1 patients were tested and the results demonstrated the feasibility of using the DBS method for clinical biomarker monitoring.
Conclusion:
The new approach greatly improves assay precision and accuracy.
