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Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

Systems for multiplexing homogeneous immunoassays.

Cameron D Yamanishi^1,2, Joyce Han-Ching Chiu^1,2, Shuichi Takayama^1,2,3,4

¹Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

|July 14, 2015

Summary

This summary is machine-generated.

Homogeneous multiplex immunoassays offer efficient biomarker discovery and drug development by minimizing sample and reagent use. This review discusses their speed, simplicity, and multiplexing capabilities, addressing challenges like signal cross-talk.

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Area of Science:

Biomarker Discovery and Development
Proteomics and Immunoassays
High-Throughput Screening Technologies

Background:

High-throughput multiplex protein biomarker assays are crucial for biomarker discovery and drug development.
Homogeneous multiplex systems offer advantages in assay speed and reagent cost-efficiency.
Challenges include signal cross-talk and biochemical cross-reactivity, hindering assay robustness.

Purpose of the Study:

To review advancements in homogeneous multiplex immunoassay platforms.
To discuss the balance between assay speed, simplicity, multiplexing capability, and robustness.
To highlight strategies for overcoming inherent challenges in homogeneous multiplex assays.

Main Methods:

Review of demonstrated homogeneous multiplex immunoassay technologies.
Analysis of techniques including fluorescent polarization, FRET with quantum dots/graphene, luminescent oxygen-channeling immunoassays with aqueous two-phase systems, and DNA proximity assays.
Discussion of the trade-offs between assay performance metrics.

Main Results:

Several homogeneous multiplex immunoassay platforms have been successfully developed.
These platforms demonstrate potential for rapid and cost-effective analysis of multiple protein biomarkers.
Key challenges such as signal interference and cross-reactivity persist but are being addressed.

Conclusions:

Homogeneous multiplex immunoassays represent a significant advancement in high-throughput biomarker analysis.
Continued research is needed to optimize robustness and overcome cross-reactivity issues.
These assays are vital for accelerating biomarker discovery and drug development pipelines.