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Updated: Apr 7, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
A new approach to detect early or hidden fungal development in indoor environments
Rukshala Anton1, Stéphane Moularat1, Enric Robine1
1Centre Scientifique et Technique du Bâtiment (CSTB), 84, Avenue Jean Jaurès Champs-sur-Marne, 77447 Marne-la-Vallée Cedex 2, France.
Abstract:
In addition to the biodegradation problems encountered in buildings, exposure of their occupants to mold is responsible for numerous diseases such as respiratory infections, immediate or delayed allergies and different types of irritations. However, current techniques are unable to detect mold at an early stage of development or hidden contaminants. Moularat et al., in 2008 has established chemical fingerprints of moldy growth from Volatile Organic Compounds (VOCs) arising specifically from fungal metabolism and developed the Fungal Contamination Index (FCI) (Moularat et al., 2008a,b). This index has the advantage of detecting fungal development both reliably and rapidly before any visible signs of contamination could be detected. However, even though the FCI has been widely tested, VOCs' analysis by GC/MS, which is required for index calculation, is incompatible with real-time monitoring strategy for indoor environments. In this context, researches around FCI exploitation have been followed up in order to provide a monitoring device widely deployable which is the result of the miniaturization of an analytical chain for portable, reliable and low-cost applications. This device is based on one hand the selection and concentration of chemical compounds from the sample of interest and on the other hand the development of an array of different conducting polymer based sensors in order to obtain a specific footprint. This fungal contamination detection device was the subject of patent applications by the CSTB. The modularity of the system (ability to vary both the elements of detection polymers and retention time of interest) allows for expansion of its use to other pollutants.
Insights
Early detection of indoor mold is crucial for health. A new device uses conducting polymer sensors to identify fungal contamination via Volatile Organic Compounds (VOCs) before visible signs appear.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- Building mold exposure causes significant health issues, including respiratory infections and allergies.
- Current detection methods fail to identify mold early or detect hidden contamination.
- Volatile Organic Compounds (VOCs) from fungal metabolism can serve as biomarkers for mold growth.
Purpose of the Study:
- To develop a portable, real-time monitoring device for early fungal contamination detection.
- To overcome the limitations of Gas Chromatography/Mass Spectrometry (GC/MS) for indoor environmental monitoring.
- To create a low-cost, reliable system for identifying mold before visible signs emerge.
Main Methods:
- Miniaturization of an analytical chain for portable applications.
- Development of an array of conducting polymer-based sensors.
- Selection and concentration of chemical compounds (VOCs) from air samples.
- Utilizing specific VOC "fingerprints" for mold identification.
Main Results:
- A prototype device for detecting fungal contamination was developed and patented.
- The system demonstrated the ability to identify mold through specific VOC profiles.
- The device's modularity allows for potential adaptation to detect other indoor pollutants.
Conclusions:
- The developed device offers a promising solution for early and reliable mold detection in indoor environments.
- This technology enables real-time monitoring, crucial for public health and building management.
- The system's adaptability suggests broader applications in environmental monitoring and pollutant detection.

