Related Experiment Video
Updated: Apr 7, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Published on: December 22, 2017
Effect of interleukin-17 on receptor-interacting protein 4 expression and keratinocyte proliferation
Kun Jia1, Yan Zhang1, Weiyuan Ma2
1Shandong University School of Medicine, Jinan, Shandong 250012, P.R. China.
Abstract:
The aim of the present study was to investigate the effect of receptor-interacting protein 4 (RIP4) on keratinocyte proliferation and its role in the pathogenesis of psoriasis vulgaris. The expression of RIP4 and Ki-67 in fixed sections from 30 patients with psoriasis vulgaris and 30 gender- and age-matched healthy controls was detected by two-step immunohistochemistry, prior to the correlation being examined with Pearson's analysis. Reverse transcription-semi-quantitative polymerase chain reaction and western blot analyses were carried out to detect the mRNA and protein expression of RIP4 in an immortalized human keratinocyte line, HaCaT, stimulated by different concentrations of interleukin-17 (IL-17), in order to analyze the change in RIP4 expression following IL-17 stimulation. The cell proliferation rate was measured using the cell counting kit-8 assay simultaneously. RIP4 was mainly present in the cytoplasm of the keratinocytes. Compared with its expression in the healthy control skin, RIP4 exhibited a significant upregulation in the psoriatic lesions (P<0.05). Pearson's correlation analysis revealed a positive correlation between the expression level of RIP4 and the proliferation index. Both RIP4 mRNA and protein levels were significantly increased following IL-17 stimulation. Exposure to IL-17 additionally increased the proliferation rate of the HaCaT cells. In conclusion, RIP4 may play a role in the pathogenesis of psoriasis vulgaris as a potential target of IL-17.
More Related Videos
08:19Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
10:34Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Published on: April 14, 2010
Related Concept Videos
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway
MAPK Signaling Cascades
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...