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A new peptide for human parvovirus B19 antibody detection
E Fridell1, J Trojnar, B Wahren
1Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Scandinavian Journal of Infectious Diseases
|January 1, 1989
Abstract:
A serological assay for human parvovirus B19 was developed. Linear overlapping synthetic peptides were synthesised according to parts of open reading frames 1 and 2. A region at the N-terminus of viral protein VP2 detected serological reactivity in indirect enzyme-linked immunosorbent assays for IgG and IgM with known seropositive human sera. A cyclized peptide taken from this region, amino acids 284-307, gave the best selective reactivity with seropositive and seronegative sera. The peptide assay appears suitable for further studies of B19 infections and their complications.